Recombinant:Article Title: Prenylated quinolinecarboxylic acid derivative suppresses immune response through inhibition of PAK2.
Article Snippet: .. Mouse anti-FLAG M2 monoclonal antibody (mAb), mouse anti-b-actin mAb, dimethyl sulfoxide (DMSO), glyceryl trioctanoate, and ConA were purchased from Sigma–Aldrich (St. Louis, MO); rabbit anti-PAK2 polyclonal antibody, rabbit antiphospho-PAK2 (Ser141) polyclonal antibody, and FK506 were obtained from Cell Signaling Technology (Beverly, MA); recombinant human histone H4 protein was from New England Biolabs (Beverly, MA); sequencing grade modified trypsin and DNase I were from Promega (Madison, WI); Imject Alum Adjuvant, human IL2 enzyme-linked immunosorbent assay (ELISA) kit, and SuperScript Reverse Transcriptase III were from Thermo Fisher Scientific (Waltham, MA); mouse IgE ELISA kit, human IL4, IL6, and TNFa ELISA kit, and Propidium iodide (PI) were from eBioscience (San Diego, CA); vaseline and Lys-C were from Wako (Tokyo, Japan); ISOGEN was from NIPPON GENE Co. (Tokyo, Japan); c[32P]ATP was from PerkinElmer (Waltham, MA); horseradish peroxidaseconjugated anti-mouse IgM, IgG1, or IgG3 antibodies were from SouthernBiotech (Birmingham, AL); SureBlue was from KPL (Gaithersburg, MD); (4-hydroxy-3-nitrophenyl)acetyl-conjugated chicken c-globulin (NP-CGG) and NP-bovine serum albumin (NPBSA) were from Biosearch Technologies (Petaluma, CA); Immobilized pH gradient (IPG) gel strips (pI 4–7, pI 3–10, 18 cm) were from GE Healthcare (Waukesha, WI). ..
Sequencing:Article Title: Prenylated quinolinecarboxylic acid derivative suppresses immune response through inhibition of PAK2.
Article Snippet: .. Mouse anti-FLAG M2 monoclonal antibody (mAb), mouse anti-b-actin mAb, dimethyl sulfoxide (DMSO), glyceryl trioctanoate, and ConA were purchased from Sigma–Aldrich (St. Louis, MO); rabbit anti-PAK2 polyclonal antibody, rabbit antiphospho-PAK2 (Ser141) polyclonal antibody, and FK506 were obtained from Cell Signaling Technology (Beverly, MA); recombinant human histone H4 protein was from New England Biolabs (Beverly, MA); sequencing grade modified trypsin and DNase I were from Promega (Madison, WI); Imject Alum Adjuvant, human IL2 enzyme-linked immunosorbent assay (ELISA) kit, and SuperScript Reverse Transcriptase III were from Thermo Fisher Scientific (Waltham, MA); mouse IgE ELISA kit, human IL4, IL6, and TNFa ELISA kit, and Propidium iodide (PI) were from eBioscience (San Diego, CA); vaseline and Lys-C were from Wako (Tokyo, Japan); ISOGEN was from NIPPON GENE Co. (Tokyo, Japan); c[32P]ATP was from PerkinElmer (Waltham, MA); horseradish peroxidaseconjugated anti-mouse IgM, IgG1, or IgG3 antibodies were from SouthernBiotech (Birmingham, AL); SureBlue was from KPL (Gaithersburg, MD); (4-hydroxy-3-nitrophenyl)acetyl-conjugated chicken c-globulin (NP-CGG) and NP-bovine serum albumin (NPBSA) were from Biosearch Technologies (Petaluma, CA); Immobilized pH gradient (IPG) gel strips (pI 4–7, pI 3–10, 18 cm) were from GE Healthcare (Waukesha, WI). ..
Modification:Article Title: Prenylated quinolinecarboxylic acid derivative suppresses immune response through inhibition of PAK2.
Article Snippet: .. Mouse anti-FLAG M2 monoclonal antibody (mAb), mouse anti-b-actin mAb, dimethyl sulfoxide (DMSO), glyceryl trioctanoate, and ConA were purchased from Sigma–Aldrich (St. Louis, MO); rabbit anti-PAK2 polyclonal antibody, rabbit antiphospho-PAK2 (Ser141) polyclonal antibody, and FK506 were obtained from Cell Signaling Technology (Beverly, MA); recombinant human histone H4 protein was from New England Biolabs (Beverly, MA); sequencing grade modified trypsin and DNase I were from Promega (Madison, WI); Imject Alum Adjuvant, human IL2 enzyme-linked immunosorbent assay (ELISA) kit, and SuperScript Reverse Transcriptase III were from Thermo Fisher Scientific (Waltham, MA); mouse IgE ELISA kit, human IL4, IL6, and TNFa ELISA kit, and Propidium iodide (PI) were from eBioscience (San Diego, CA); vaseline and Lys-C were from Wako (Tokyo, Japan); ISOGEN was from NIPPON GENE Co. (Tokyo, Japan); c[32P]ATP was from PerkinElmer (Waltham, MA); horseradish peroxidaseconjugated anti-mouse IgM, IgG1, or IgG3 antibodies were from SouthernBiotech (Birmingham, AL); SureBlue was from KPL (Gaithersburg, MD); (4-hydroxy-3-nitrophenyl)acetyl-conjugated chicken c-globulin (NP-CGG) and NP-bovine serum albumin (NPBSA) were from Biosearch Technologies (Petaluma, CA); Immobilized pH gradient (IPG) gel strips (pI 4–7, pI 3–10, 18 cm) were from GE Healthcare (Waukesha, WI). ..
Adjuvant:Article Title: Prenylated quinolinecarboxylic acid derivative suppresses immune response through inhibition of PAK2.
Article Snippet: .. Mouse anti-FLAG M2 monoclonal antibody (mAb), mouse anti-b-actin mAb, dimethyl sulfoxide (DMSO), glyceryl trioctanoate, and ConA were purchased from Sigma–Aldrich (St. Louis, MO); rabbit anti-PAK2 polyclonal antibody, rabbit antiphospho-PAK2 (Ser141) polyclonal antibody, and FK506 were obtained from Cell Signaling Technology (Beverly, MA); recombinant human histone H4 protein was from New England Biolabs (Beverly, MA); sequencing grade modified trypsin and DNase I were from Promega (Madison, WI); Imject Alum Adjuvant, human IL2 enzyme-linked immunosorbent assay (ELISA) kit, and SuperScript Reverse Transcriptase III were from Thermo Fisher Scientific (Waltham, MA); mouse IgE ELISA kit, human IL4, IL6, and TNFa ELISA kit, and Propidium iodide (PI) were from eBioscience (San Diego, CA); vaseline and Lys-C were from Wako (Tokyo, Japan); ISOGEN was from NIPPON GENE Co. (Tokyo, Japan); c[32P]ATP was from PerkinElmer (Waltham, MA); horseradish peroxidaseconjugated anti-mouse IgM, IgG1, or IgG3 antibodies were from SouthernBiotech (Birmingham, AL); SureBlue was from KPL (Gaithersburg, MD); (4-hydroxy-3-nitrophenyl)acetyl-conjugated chicken c-globulin (NP-CGG) and NP-bovine serum albumin (NPBSA) were from Biosearch Technologies (Petaluma, CA); Immobilized pH gradient (IPG) gel strips (pI 4–7, pI 3–10, 18 cm) were from GE Healthcare (Waukesha, WI). ..
Enzyme-linked Immunosorbent Assay:Article Title: Prenylated quinolinecarboxylic acid derivative suppresses immune response through inhibition of PAK2.
Article Snippet: .. Mouse anti-FLAG M2 monoclonal antibody (mAb), mouse anti-b-actin mAb, dimethyl sulfoxide (DMSO), glyceryl trioctanoate, and ConA were purchased from Sigma–Aldrich (St. Louis, MO); rabbit anti-PAK2 polyclonal antibody, rabbit antiphospho-PAK2 (Ser141) polyclonal antibody, and FK506 were obtained from Cell Signaling Technology (Beverly, MA); recombinant human histone H4 protein was from New England Biolabs (Beverly, MA); sequencing grade modified trypsin and DNase I were from Promega (Madison, WI); Imject Alum Adjuvant, human IL2 enzyme-linked immunosorbent assay (ELISA) kit, and SuperScript Reverse Transcriptase III were from Thermo Fisher Scientific (Waltham, MA); mouse IgE ELISA kit, human IL4, IL6, and TNFa ELISA kit, and Propidium iodide (PI) were from eBioscience (San Diego, CA); vaseline and Lys-C were from Wako (Tokyo, Japan); ISOGEN was from NIPPON GENE Co. (Tokyo, Japan); c[32P]ATP was from PerkinElmer (Waltham, MA); horseradish peroxidaseconjugated anti-mouse IgM, IgG1, or IgG3 antibodies were from SouthernBiotech (Birmingham, AL); SureBlue was from KPL (Gaithersburg, MD); (4-hydroxy-3-nitrophenyl)acetyl-conjugated chicken c-globulin (NP-CGG) and NP-bovine serum albumin (NPBSA) were from Biosearch Technologies (Petaluma, CA); Immobilized pH gradient (IPG) gel strips (pI 4–7, pI 3–10, 18 cm) were from GE Healthcare (Waukesha, WI). ..
Reverse Transcription:Article Title: Prenylated quinolinecarboxylic acid derivative suppresses immune response through inhibition of PAK2.
Article Snippet: .. Mouse anti-FLAG M2 monoclonal antibody (mAb), mouse anti-b-actin mAb, dimethyl sulfoxide (DMSO), glyceryl trioctanoate, and ConA were purchased from Sigma–Aldrich (St. Louis, MO); rabbit anti-PAK2 polyclonal antibody, rabbit antiphospho-PAK2 (Ser141) polyclonal antibody, and FK506 were obtained from Cell Signaling Technology (Beverly, MA); recombinant human histone H4 protein was from New England Biolabs (Beverly, MA); sequencing grade modified trypsin and DNase I were from Promega (Madison, WI); Imject Alum Adjuvant, human IL2 enzyme-linked immunosorbent assay (ELISA) kit, and SuperScript Reverse Transcriptase III were from Thermo Fisher Scientific (Waltham, MA); mouse IgE ELISA kit, human IL4, IL6, and TNFa ELISA kit, and Propidium iodide (PI) were from eBioscience (San Diego, CA); vaseline and Lys-C were from Wako (Tokyo, Japan); ISOGEN was from NIPPON GENE Co. (Tokyo, Japan); c[32P]ATP was from PerkinElmer (Waltham, MA); horseradish peroxidaseconjugated anti-mouse IgM, IgG1, or IgG3 antibodies were from SouthernBiotech (Birmingham, AL); SureBlue was from KPL (Gaithersburg, MD); (4-hydroxy-3-nitrophenyl)acetyl-conjugated chicken c-globulin (NP-CGG) and NP-bovine serum albumin (NPBSA) were from Biosearch Technologies (Petaluma, CA); Immobilized pH gradient (IPG) gel strips (pI 4–7, pI 3–10, 18 cm) were from GE Healthcare (Waukesha, WI). ..
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